Older PCR protocol

You should first run qPCR to adjust template concentration (see real-time PCR). 4X linear PCR: Reagent Initial Final 1X reaction (50 μl) 4.5X (μl) Kapa Buffer A 10x 1x 5 22.5 Forward 10 µM 0.4 µM 2 9 Reverse (T7) 10 µM 0.4 µM 2 9 dNTP mix 10 mM 0.4 mM 2 9 Raw library 2 ng/µL 0.2 ng/µLContinue reading “Older PCR protocol”


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