Cell Culture

Cell lines: 1. IMR90 (Human fibroblasts) 2. PGP1 (Hu iPSCs) For culturing Hu iPSC (PGP1) I use  Wicell Feeder–Independent Pluripotent Stem Cell Protocols mTeSR™1 Medium. 3. PGP1 primary fibroblasts (PGP1F) Freezing IMR90 cells (Caroline) Make freezing media 90% FBS and 10% DMSO. For example, 25 ml FBS, 2.78 ml DMSO. Stable in 4c for about a week.Continue reading “Cell Culture”