Cleaning MatTeks

It is recommended to clean the MatTek bottom, especially for super-resolution/single-molecule imaging. I use BRANSON  ultrasonic cleaner 1800.

1. make 1:12 detergent solution from the BRANSON cleaning solution (or Alconox, etc). I add 25 ml 1:12 cleaning solution to 275 ml deionized water.
2. Degas for 5 minutes.
3. Place MatTeks (only the bottom is enough) inside a beaker with the cleaning solution and sonicate for 10 minutes.
4. Place beaker with deionized water and degas for 5 minutes. In the meanwhile, rinse MatTeks with deionized water and temporary place in another beaker with deionized water.
5. Sonicate MatTeks for 10 minutes in in the degassed, deionized water.
6. Drain and lightly spray with Ethanol. Drain and place in tissue culture hood.