Updated 08/08/22 Linear PCR: pre-amplification of the entire library Twist recommends 0.4 ng/µL template concentration. 2X linear PCR: Reagent Initial Final 1X reaction (50 μl) 2X (μl) Roche Diagnostics KAPA HiFi HotStart ReadyMix PCR Kit (6.25 mL), 250 x 50 uL reactions 2x 1x 25 50 Forward 10 µM 0.3 µM 1.5 3 Reverse (with T7 promoter) 10 µMContinue reading “Probe amplification using T7”
