Updated 08/08/22 Linear PCR: pre-amplification of the entire library Twist recommends 0.4 ng/µL template concentration. 2X linear PCR: Reagent Initial Final 1X reaction (50 μl) 2X (μl) Roche Diagnostics KAPA HiFi HotStart ReadyMix PCR Kit (6.25 mL), 250 x 50 uL reactions 2x 1x 25 50 Forward 10 µM 0.3 µM 1.5 3 Reverse (with T7 promoter) 10 µMContinue reading “Probe amplification using T7”
Tag Archives: T7
Online Notebook!
I would like to manage a mostly open-access e-notebook and share my findings! My inspiration for that idea comes from Alistair Boettiger’s notebook. 06/07/2017 Walk today: IMR90 Cells. 04/18/2017 18th run: FISH wasn’t great, but found a reasonable cell. Had bubbles. Can first see them at the pipe coming out of valve 0, and heading toContinue reading “Online Notebook!”